Reaction mixture containing 0,1 µM/mL CdTe quantum dots, 2 mg/mL protein, 1 mg/mL sulfo-NHS (CAS# 106627-54-7), 10 mg/mL EDC (CAS# 25952-53-8) in pH 7,0 PBS buffer is prepared and stored for 2-4 h at room temperature and then stored at 4°C overnight.
The precipitate (unconjugated Q-dots) if any is removed by centrifugation. The stock of ready-to-use product should be stored at 4°C. Optionally it can be dialyzed on a memb-rane with MWCO of 12000-14000 against pH 7,0 PBS buffer and stored at 4°C. Emission wavelength may slightly shift after labeling procedure.